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Competition

Epitope mapping and specificity measurements are essential for characterization of new ligands. With TraceDrawer you can simultaneously quantify the binding properties of two molecules competing for the same target and evaluate how much the presence of the competitor influences the interaction.

Competition measurement

Although only one of the binders can produce a signal, the affinity and kinetics of both the fluorescently labeled antibody and the non-labeled competitor (EGF) can be characterized through curve fitting.

Key features

  • Estimate interaction properties of competing molecules
  • Investigate self-competition
  • Work with labeled/non-labeled compounds
  • Use MW differences to separate competitors in label-free systems